Seroprevalence and newly isolated strains of LT-Ag gene (MMGHA2 and MM GHA5) of BK Polyomavirus in A Sample of Iraqi renal transplant recipients

Background: BK virus (BKV) opportunistic viral infection causes nephropathy and allograft loss in renal transplant recipients (RTRs).Aims: Investigating BKV seropositivity, viremia rate function were the primary goals of this study, addition to molecular study Iraqi strains (LT antigen gene).Settings Design: Case-control study.material methods: Serum plasma specimens collected from 106 RTR 100 non-RTR. samples analyzed for anti-BK IgG antibodies by BK-IgG ELISA kit. Plasma used detection large tumor (LT-Ag) gene region RT-PCR technique then sequenced Sanger's method.Statistical analysis: frequencies, percentages, Chi square-test SPSS v.28Results: The Study showed that 114 (55.3%) have a positive result BK-IgG, so there was no significant difference between seropositivity antibody among studied groups, p =0.191. On other hand, total 206 subjects, 31 (15.0%) had 175 (85.0%) negative. Renal highly differences (p ≤0.000) seropositive negative patients. 2 sequences local deposited GenBank with accession numbers LC718551.1 LC718552.1Conclusions: viremic state has similarly impact on function. While association high levels serum creatinine. New found patients 98% similarity reference gene.